JOMPAC
Journal of Medicine and Palliative Care

Journal of Medicine and Palliative Care (JOMPAC) is an open access scientific journal with independent, unbiased, and double-blind review under international guidelines. The purpose of JOMPAC is to contribute to the literature by publishing articles on health sciences and medicine.

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Original Article
Efficacy of vitamins B9, C, and E on fat graft viability
Abstract
Aims: This study aims to investigate the potential of folic acid (vitamin B9), ascorbic acid (vitamin C), and ?-tocopherol (vitamin E) in enhancing the survival of fat grafts.
Methods: We divided the dorsal regions of ten Sprague-Dawley rats into four quadrants, serving as recipient sites for inguinal fat grafts. Fat grafts were placed in these sites after incubation, with different sites receiving 0.9% NaCl (left cranial), vitamin B9 (right cranial), vitamin C (left caudal), and vitamin E (right caudal). After three months, we harvested the fat grafts and evaluated their viability using perilipin immunohistochemistry.
Results: Folic acid, vitamin C, and vitamin E all significantly improved fat graft survival rates when compared with control (p<0.001 for each).
Conclusion: Folic acid (vitamin B9) promotes angiogenesis and collagen synthesis, vitamin C’s antioxidant properties support collagen synthesis, and vitamin E’s potent antioxidant capacity protects grafted adipose tissue from oxidative stress and inflammation, facilitating graft vascularization and integration. These findings suggest that these readily available and cost-effective vitamins, B9, C, and E, enhance fat graft survival immunohistochemically. The improved graft viability presented here could inspire further clinical studies and applications.

Keywords : Adipogenesis, ascorbic acid, fat graft survival, folic acid, vitamin C, vitamin E

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1. Trotzier C, Sequeira I, Auxenfans C, Mojallal AA. Fat graftretention: adipose tissue, adipose-derived stem cells, and aging.Plast Reconstr Surg. 2023;151(3):420e-431e.
2. Kelly KB, Kennelly JP, Ordonez M, et al. Excess folic acid increaseslipid storage, weight gain, and adipose tissue inflammation inhigh fat diet-fed rats. Nutrients. 2016;8(10):594.
3. Lunger A, Ismail T, Todorov A, et al. Improved adipocyte viabilityin autologous fat grafting with ascorbic acid-supplementedtumescent solution. Ann Plast Surg. 2019;83(4):464-467.
4. Tanaka-Yachi R, Takahashi-Muto C, Adachi K, et al. Promotingeffect of &alpha;-tocopherol on beige adipocyte differentiation in 3T3-L1 cells and rat white adipose tissue. J Oleo Sci. 2017;66(2):171-179. Erratum in: J Oleo Sci. 2019;68(8):809.
5. Okyay MF, K&ouml;m&uuml;rc&uuml; H, Bağhaki S, Demir&ouml;z A, Aydın &Ouml;, ArslanH. Effects of insulin, metoprolol and deferoxamine on fat graftsurvival. Aesthetic Plast Surg. 2019;43(3):845-852.
6. Pham NA, Morrison A, Schwock J, et al. Quantitative imageanalysis of immunohistochemical stains using a CMYK colormodel. Diagn Pathol. 2007;2(1):1-10.
7. Karakol P, Bozkurt M, Gelbal C, et al. Efficacy of stromal vascularfraction and enzyme-free mechanical isolation therapy inexperimental full thickness burn wounds. J Plast Surg Hand Surg.2023;57(1-6):78-94.
8. Lunger A, Ismail T, Todorov A, et al. Improved adipocyte viabilityin autologous fat grafting with ascorbic acid-supplementedtumescent solution. Ann Plast Surg. 2019;83(4):464-467.
9. Abbas DB, Lavin CV, Fahy EJ, et al. Fat grafts augmented withvitamin E improve volume retention and radiation-inducedfibrosis. Aesthet Surg J. 2022;42(8):946-955.
10. Cinar F, Yalcin CE, Ayas G, et al. Increased total antioxidantcapacity value improves survival of fat grafts in a rat model. PlastReconstr Surg. 2023;10:1097. doi: 10.1097/PRS.0000000000010873
11. Nemir S, Hanson SE, Chu CK. Surgical decision making inautologous fat grafting: an evidence-based review of techniquesto maximize fat survival. Aesthet Surg J. 2021;41(Suppl 1):S3-S15.
12. Pu LLQ. Fat Grafting for facial rejuvenation: my preferredapproach. Clin Plast Surg. 2020;47(1):19-29.
13. Shih L, Davis MJ, Winocour SJ. The science of fat grafting. SeminPlast Surg. 2020;34(1):5-10.
14. Loder S, Wang S, Amurgis C, et al. Active vitamin D3 (calcitriol)increases adipose graft retention in a xenograft model. AesthetSurg J. 2023;43(6):NP449-NP465.
15. Wu W, Bi X, Zhao J, et al. Ultra-condensed fat: a novel fat productfor volume augmentation. Aesthetic Plast Surg. 2023;47(5):2074-2083.
16. Khouri RK Jr, Khouri RK. Current clinical applications of fatgrafting. Plast Reconstr Surg. 2017;140(3):466e-486e.
17. Deptula P, Fox P. Autologous fat grafting in hand surgery. J HandSurg Am. 2021;46(7):594-600.
18. Prescher H, Froimson JR, Hanson SE. Deconstructing fat toreverse radiation induced soft tissue fibrosis. Bioengineering(Basel). 2023;10(6):742.
19. Cond&eacute;-Green A, Wu I, Graham I, et al. Comparison of 3techniques of fat grafting and cell-supplemented lipotransfer inathymic rats: a pilot study. Aesthet Surg J. 2013;33(5):713-721.
20. Guerrerosantos J, Gonzalez-Mendoza A, Masmela Y, GonzalezMA, Deos M, Diaz P. Long-term survival of free fat grafts inmuscle: an experimental study in rats. Aesthetic Plast Surg.1996;20(5):403-408.
Article available in :
Volume 4, Issue 5, 2023
Page : 561-565
https://doi.org/10.47582/jompac.1359372
Article Information
Received : 13 Eyl 2023
Accepted : 13 Eki 2023
Published : 27 Eki 2023
Corresponding Author :

Mehmet Fatih Okyay: Plastic, Reconstructive and Aesthetic Surgery Specialist, MFO Clinic, Private Practice, Antalya, Turkey

[email protected]
Citation : Okyay MF. Efficacy of vitamins B9, C, and E on fat graft viability. J Med Palliat Care. 2023;4(5):561-565.

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